Distribution of galanin receptor 2b neurons and interaction with galanin in the zebrafish central nervous system. (15th August 2016)

• Record Type: Journal Article
• Authors: Kim, Eunmi; Jeong, Inyoung; Kim, Suhyun; Kim, Hwan-Ki; Lee, Dong-Won; Kim, Boa; Seong, Jae Young; Bae, Young-Ki; Ryu, Jae-Ho; Park, Hae-Chul
• Is Part Of: Neuroscience letters. Volume 628(2016)
• Page Start: 153
• Page End: 160
• Publication Date: 2016-08-15
• Subjects: Neuropeptide -- Galanin -- Galanin receptor -- Central nervous system -- Zebrafish; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2016.06.025
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: A novel galanin receptor 2b ( galr2b ) is expressed in the zebrafish nervous system. The galr2b:egfp transgenic zebrafish was generated to visualize galr2b neurons. Distribution of galr2b neurons and their interaction with galanin was analyzed in the zebrafish central nervous system. Abstract: Galanin is a multifunctional neuropeptide that is implicated in the modulation of physiological processes, including nociception, cognition, feeding behavior, neuronal growth, and reproduction. The physiological effects of galanin are mediated through its interaction with three different G protein-coupled receptors, i.e., GALR1, GALR2, and GALR3. Unlike mammals, zebrafish have four different receptors for galanin, diversified from GALR1 (GAL1a and GALR1b) and GALR2 (GALR2a and GALR2b). Despite the importance of galanin in the central nervous system (CNS), no information has been reported regarding GalR2 in zebrafish CNS. In this study, we found that galr2a is expressed at low levels in restricted areas of the brain; however, galr2b was widely expressed in CNS including olfactory bulb, midbrain tegmentum, preoptic region, dorsal thalamus, posterior tuberculum, postoptic commissure, hindbrain, and spinal cord. To further analyze the distribution of GALR2b neurons and their interaction with GAL, we generated Tg(galr2b:egfp) zebrafish, which express enhanced green fluorescent protein (EGFP) under the control of a galr2b promoter. Investigation of the CNS of transgenic reporter zebrafish revealed that galr2b :EGFP + neurons are distributed and interact with galanin-immunoreactive (galanin-IR) cells in various regions of the brain and spinal cord. We found that in some regions of the brain and spinal cord, galanin-IR nerve cells were not observed near galr2b :EGFP neurons, suggesting that GALR2b may have the potential to interact with other ligands instead of galanin in these regions.