Depletion of tyrosyl‐DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula. Issue 7 (24th February 2016)

• Record Type: Journal Article
• Authors: Sabatini, Maria Elisa; Donà, Mattia; Leonetti, Paola; Minio, Andrea; Delledonne, Massimo; Carboneral, Daniela; Confalonieri, Massimo; Giraffa, Giorgio; Balestrazzi, Alma
• Is Part Of: Journal of integrative plant biology. Volume 58:Issue 7(2016)
• Page Start: 618
• Page End: 622
• Publication Date: 2016-02-24
• Subjects: DNA methylation -- Medicago truncatula -- RNA‐Seq -- transposable elements -- tyrosyl‐DNA phosphodiesterase; Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.12457
• Languages: English
• ISSNs: 1672-9072

Abstract

Summary: The role of plant tyrosyl‐DNA phosphodiesterase 1α in genome stability is studied using a Medicago truncatula MtTdp1α ‐depleted line. Lack of MtTdp1α results in a 39% reduction of methylated cytosines as compared to control. RNA‐Seq analyses revealed that 11 DNA transposons and 22 retrotransposons were differentially expressed in the Tdp1α‐2a line. Among them all, DNA transposons ( MuDR, hAT, DNA3‐11_Mad ) and seven retrotransposons (LTR (Long Terminal Repeat)/ Gipsy, LTR/ Copia, LTR and NonLTR/L1) were down‐regulated, while the 15 retrotransposons were up‐regulated. Results suggest that the occurrence of stress‐responsive cis ‐elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.