Heat shock transcription factor 1‐associated expression of slow myosin heavy chain in mouse soleus muscle in response to unloading with or without reloading. (9th May 2016)

• Record Type: Journal Article
• Authors: Yokoyama, S.; Ohno, Y.; Egawa, T.; Yasuhara, K.; Nakai, A.; Sugiura, T.; Ohira, Y.; Yoshioka, T.; Okita, M.; Origuchi, T.; Goto, K.
• Is Part Of: Acta physiologica. Volume 217:Number 4(2016)
• Page Start: 325
• Page End: 337
• Publication Date: 2016-05-09
• Subjects: glycogen synthase kinase 3 -- heat shock transcription factor 1 -- microRNA -- myosin heavy chain -- nuclear factor of activated T cells; Physiology -- Periodicals; Physiology -- Research -- Periodicals; 612
• DOI: 10.1111/apha.12692
• Languages: English
• ISSNs: 1748-1708

Abstract

Abstract: Aim: The effects of heat shock transcription factor 1 (HSF1) deficiency on the fibre type composition and the expression level of nuclear factor of activated T cells (NFAT) family members (NFATc1, NFATc2, NFATc3 and NFATc4), phosphorylated glycogen synthase kinase 3 α (p‐GSK3 α ) and p‐GSK3 β, microRNA‐208b (miR‐208b), miR‐499 and slow myosin heavy chain (MyHC) mRNAs (Myh7 and Myh7b) of antigravitational soleus muscle in response to unloading with or without reloading were investigated. Methods: HSF1‐null and wild‐type mice were subjected to continuous 2‐week hindlimb suspension followed by 2‐ or 4‐week ambulation recovery. Results: In wild‐type mice, the relative population of slow type I fibres, the expression level of NFATc2, p‐GSK3 ( α and β ), miR‐208b, miR‐499 and slow MyHC mRNAs (Myh7 and Myh7b) were all decreased with hindlimb suspension, but recovered after it. Significant interactions between train and time (the relative population of slow type I fibres; P = 0.01, the expression level of NFATc2; P = 0.001, p‐GSK β ; P = 0.009, miR‐208b; P = 0.002, miR‐499; P = 0.04) suggested that these responses were suppressed in HSF1‐null mice. Conclusion: HSF1 may be a molecule in the regulation of the expression of slow MyHC as well as miR‐208b, miR‐499, NFATc2 and p‐GSK3 ( α and β ) in mouse soleus muscle.