Priming mobilized peripheral blood mononuclear cells with the "activated platelet supernatant" enhances the efficacy of cell therapy for myocardial infarction of rats. Issue 4 (August 2016)

• Record Type: Journal Article
• Authors: Kang, Jeehoon; Hur, Jin; Kang, Jin‐A; Lee, Hak Seung; Jung, Heewon; Choi, Jae‐Il; Lee, Hwan; Kim, Yong Sook; Ahn, Youngkeun; Kim, Hyo‐Soo
• Is Part Of: Cardiovascular therapeutics. Volume 34:Issue 4(2016:Aug.)
• Page Start: 245
• Page End: 253
• Publication Date: 2016-08
• Subjects: Activated platelet supernatant -- Cell priming -- Cell Therapy -- Granulocyte colony‐stimulating factor‐mobilized peripheral blood mononuclear cells -- M2 polarization -- Myocardial infarction; Cardiovascular pharmacology -- Periodicals; Cardiovascular agents -- Periodicals; Cardiovascular system -- Diseases -- Chemotherapy -- Periodicals; Cardiovascular Agents -- Periodicals; Cardiovascular Diseases -- drug therapy -- Periodicals; Agents cardiovasculaires -- Périodiques; Appareil cardiovasculaire -- Maladies -- Chimiothérapie -- Périodiques; 616.1005
• DOI: 10.1111/1755-5922.12194
• Languages: English
• ISSNs: 1755-5914

Abstract

Summary: Aim: Various methods are used to augment the efficacy of cell therapy in myocardial infarction (MI). In this study, we used the "activated platelet supernatant (APS)" to prime autologous "granulocyte colony‐stimulating factor‐mobilized peripheral blood mononuclear cells ( mob PBMCs)" and investigated the efficacy of cell‐based therapy in MI. Method: Rat mob PBMCs were isolated after daily subcutaneous injections of G‐CSF at 100 μg/kg for 3 days. APS was isolated separately after activating rat platelets with thrombin 0.5 U/mL for 2 hours. Priming was performed with APS for 6 hours. To check the paracrine effect of primed mob PBMCs, we used the 36‐hour culture supernatant of the primed cells. A rat MI model was used for an in vivo model. Result: Cytokines such as IL‐1β, IL‐10, and TGFβ were 3.7±0.9‐fold, 3.4±1.2‐fold, and 1.2±0.1‐fold higher in APS, respectively, compared with naïve platelet supernatant. By APS priming, mob PBMCs showed M2 polarization and upregulation of angiogenic molecules (i.e., TEK, IL‐10, CXCL1, and CX3CR1). APS‐primed mob PBMCs had a 2.3‐fold increased adhesion ability, induced by upregulated integrins. Rat endothelial cells cultured in the 36‐hour culture supernatant of APS‐primed mob PBMCs showed a 1.6‐fold augmented proliferation and capillary network formation. In vivo transplantation of APS‐primed mob PBMCs into rat MI models showed a significant trend of reduction in fibrosis area ( P =.001) and wall thinning ( P =.030), which lead to improvement in cardiac function measured by echocardiography. Conclusion: Our data reveal that APS priming can enhance the wound‐healing potential of mob PBMCs. APS priming may be a promising method for cell‐based therapy of MI.