MALDI mass spectrometry imaging: A cutting‐edge tool for fundamental and clinical histopathology. Issue 7 (16th June 2016)

• Record Type: Journal Article
• Authors: Longuespée, Rémi; Casadonte, Rita; Kriegsmann, Mark; Pottier, Charles; Picard de Muller, Gaël; Delvenne, Philippe; Kriegsmann, Jörg; De Pauw, Edwin
• Is Part Of: Proteomics. Volume 10:Issue 7(2016)
• Page Start: 701
• Page End: 719
• Publication Date: 2016-06-16
• Subjects: Histology -- Imaging -- MALDI -- Mass spectrometry -- Omics -- Pathology; Proteomics -- Periodicals; 572.605
• DOI: 10.1002/prca.201500140
• Languages: English
• ISSNs: 1862-8346

Abstract

Abstract : Histopathological diagnoses have been done in the last century based on hematoxylin and eosin staining. These methods were complemented by histochemistry, electron microscopy, immunohistochemistry (IHC), and molecular techniques. Mass spectrometry (MS) methods allow the thorough examination of various biocompounds in extracts and tissue sections. Today, mass spectrometry imaging (MSI), and especially matrix‐assisted laser desorption ionization (MALDI) imaging links classical histology and molecular analyses. Direct mapping is a major advantage of the combination of molecular profiling and imaging. MSI can be considered as a cutting edge approach for molecular detection of proteins, peptides, carbohydrates, lipids, and small molecules in tissues. This review covers the detection of various biomolecules in histopathological sections by MSI. Proteomic methods will be introduced into clinical histopathology within the next few years.