Cytokine responses to eye spray adjuvants for enhancing vaccine‐induced immunity in chickens. (July 2016)

• Record Type: Journal Article
• Authors: Takaki, Hiromi; Sato, Haruko; Kurata, Riho; Hikono, Hirokazu; Hiono, Takahiro; Kida, Hiroshi; Matsumoto, Misako; Saito, Takehiko; Seya, Tsukasa
• Is Part Of: Microbiology and immunology. Volume 60:Number 7(2016:Jul.)
• Page Start: 511
• Page End: 515
• Publication Date: 2016-07
• Subjects: Chicken -- Toll‐like receptors -- vaccine adjuvant; Microbiology -- Periodicals; Immunology -- Periodicals; Allergy and Immunology -- Periodicals; Microbiology -- Periodicals; Microbiologie -- Périodiques; Immunologie -- Périodiques; 579
• DOI: 10.1111/1348-0421.12391
• Languages: English
• ISSNs: 0385-5600

Abstract

ABSTRACT: Eye spray influenza vaccines for chickens are increasingly available; however, how to enhance cellular and antibody responses to them remains undetermined. Here, eye‐drops containing the immune‐enhancing adjuvants Pam2CSK4 or polyI:C were assessed in chickens. Application of these TLR agonists to chicken conjunctiva resulted in up‐regulation of IL‐1β, but not other cytokines, including IFN and IL‐6, in the spleen, lung and Harderian gland. Thus, responses to adjuvant applied to the conjunctival mucosa of chickens differ from those expected from the responses to intra‐nasal adjuvants in mammals. Identifying an appropriate delivery route for adjuvants is crucial for evoking immune responses in chickens.