The Age of Cortical Neural Networks Affects Their Interactions with Magnetic Nanoparticles. Issue 26 (27th May 2016)

• Record Type: Journal Article
• Authors: Tay, Andy; Kunze, Anja; Jun, Dukwoo; Hoek, Eric; Di Carlo, Dino
• Is Part Of: Small. Volume 12:Issue 26(2016)
• Page Start: 3559
• Page End: 3567
• Publication Date: 2016-05-27
• Subjects: age -- CCL11/eotaxin -- conditioned media -- cortical neurons -- nanoparticles; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201600673
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract : Despite increasing use of nanotechnology in neuroscience, the characterization of interactions between magnetic nanoparticles (MNPs) and primary cortical neural networks remains underdeveloped. In particular, how the age of primary neural networks affects MNP uptake and endocytosis is critical when considering MNP‐based therapies for age‐related diseases. Here, primary cortical neural networks are cultured up to 4 weeks and with CCL11/eotaxin, an age‐inducing chemokine, to create aged neural networks. As the neural networks are aged, their association with membrane‐bound starch‐coated ferromagnetic nanoparticles (fMNPs) increases while their endocytic mechanisms are impaired, resulting in reduced internalization of chitosan‐coated fMNPs. The age of the neurons also negates the neuroprotective effects of chitosan coatings on fMNPs, attributing to decreased intracellular trafficking and increased colocalization of MNPs with lysosomes. These findings demonstrate the importance of age and developmental stage of primary neural cells when developing in vitro models for fMNP therapeutics targeting age‐related diseases. Abstract : The age of neurons (either induced with chemokine CCL11 or cultured for up to 3 weeks) affects the endocytic uptake of chitosan‐coated magnetic nanoparticles, with impairment of clathrin‐mediated endocytosis increasing with age. The neuro‐protective effects of chitosan coatings on magnetic nanoparticles are also age‐dependent due to reduced intracellular trafficking of magnetic nanoparticles with age.