Adipose stromal cells improve healing of vocal fold scar: Morphological and functional evidences. (14th April 2016)

• Record Type: Journal Article
• Authors: de Bonnecaze, Guillaume; Chaput, Benoit; Woisard, Virginie; Uro‐Coste, Emmanuelle; Swider, Pascal; Vergez, Sebastien; Serrano, Elie; Casteilla, Louis; Planat‐Benard, Valerie
• Is Part Of: Laryngoscope. Volume 126:Number 8(2016:Aug.)
• Page Start: E278
• Page End: E285
• Publication Date: 2016-04-14
• Subjects: Wound healing -- cytotherapy -- vocal fold scar -- adipose stromal cells -- biomechanical analysis; Otolaryngology -- Periodicals; 617.51005
• DOI: 10.1002/lary.25867
• Languages: English
• ISSNs: 0023-852X

Abstract

Abstract : Objectives/Hypothesis: Adipose derived stromal cells (ASCs) are abundant and easy to prepare. Such cells may be useful for treating severe vocal disturbance caused by acute vocal fold scars. Study Design: Prospective animal experiments with controls. Methods: Twenty New‐Zealand white rabbits were used in the present study. We evaluated vocal fold healing, with or without injection of autologous ASCs, after acute scarring. A defined lesion was created and the ASCs were immediately injected. Vocal fold regeneration was evaluated histomorphometrically and via viscoelastic analysis using an electrodynamic shaker. Results: Six weeks after ASC injection, vocal folds exhibited significantly less inflammation than control folds ( P < 0.005). In addition, hypertrophy of the lamina propria and fibrosis were significantly reduced upon ASC injection ( P < 0.02). The decrease in viscoelastic parameters was less important in the ASC injected group compared to the noninjected group ( P = 0.08). Conclusion: Injection of autologous ASCs improved vocal fold healing in our preclinical model. Further studies are needed, but this method may be useful in humans. Level of Evidence: NA. Laryngoscope, 126:E278–E285, 2016