Alkyl hydroperoxide reductase from Bacillus aquimaris MKSC 6.2 protects Esherichia coli from oxidative stress. (2nd November 2015)

• Record Type: Journal Article
• Authors: Natalia, Dessy; Jumadila, Ozi; Anggraini, Irika Devi; Meutia, Febrina; Puspasari, Fernita; Hasan, Khomaini
• Is Part Of: Journal of basic microbiology. Volume 56:issue 7(2016:Jul.)
• Page Start: 834
• Page End: 837
• Publication Date: 2015-11-02
• Subjects: Oxidative stress -- Alkyl hydroperoxide -- Alkyl hydroperoxide reductase; Microbiology -- Periodicals; 579
• DOI: 10.1002/jobm.201500406
• Languages: English
• ISSNs: 0233-111X

Abstract

Abstract : Alkyl hydroperoxide reductase genes ( ahpCF ) from the soft coral associated Bacillus aquimaris MKSC6.2 have been isolated. The cloned 546 bp ahpC gene encodes a 181 amino acid residues polypeptide. The AhpC belongs to typical 2‐Cys peroxiredoxin (Prx) containing conserved peroxidatic cysteine residue (C46 ) required for hydroperoxide reduction and conserved resolving cysteine (C166 ). The isolated 1530 bp ahpF gene encodes a polypeptide of 509 amino acid residues with two conserved C128 HNC131 and C337 PHC340 catalytic residues required for reduction of oxidized‐AhpC during catalytic turnover. A survival study with Escherichia coli showed that overexpression of AhpC and AhpF resulted in a total protection against 0.16 mM t‐butyl hydroperoxide.