TF‐Test Modified: New Diagnostic Tool for Human Enteroparasitosis. Issue 4 (13th May 2015)

• Record Type: Journal Article
• Authors: Carvalho, Juliana Barboza de; Santos, Bianca Martins dos; Gomes, Jancarlo Ferreira; Suzuki, Celso Tetsuo Nagase; Hoshino Shimizu, Sumie; Falcão, Alexandre Xavier; Pierucci, Julia Cestari; Matos, Lucas Vinicius Shigaki de; Bresciani, Katia Denise Saraiva
• Is Part Of: Journal of clinical laboratory analysis. Volume 30:Issue 4(2016:Jul.)
• Page Start: 293
• Page End: 300
• Publication Date: 2015-05-13
• Subjects: TF‐Test Modified -- parasitological techniques -- laboratory diagnosis -- parasitology -- intestinal parasitosis; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.21854
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract : Intestinal parasitosis is highly prevalent worldwide, being among the main causes of illness and death in humans. Currently, laboratory diagnosis of the intestinal parasites is accomplished through manual technical procedures, mostly developed decades ago, which justifies the development of more sensitive and practical techniques. Therefore, the main objective of this study was to develop, evaluate, and validate a new parasitological technique referred to as TF‐Test Modified, in comparison to three conventional parasitological techniques: TF‐Test Conventional; Rugai, Mattos & Brisola; and Helm Test/Kato‐Katz. For this realization, we collected stool samples from 457 volunteers located in endemic areas of Campinas, São Paulo, Brazil, and statistically compared the techniques. Intestinal protozoa and helminths were detected qualitatively in 42.23% (193/457) of the volunteers by TF‐Test Modified technique, against 36.76% (168/457) by TF‐Test Conventional, 5.03% (23/457) by Helm Test/Kato‐Katz, and 4.16% (19/457) by Rugai, Mattos & Brisola. Furthermore, the new technique presented "almost perfect kappa" agreement in all evaluated parameters with 95% ( P < 0.05) of estimation. The current study showed that the TF‐Test Modified technique can be comprehensively used in the diagnosis of intestinal protozoa and helminths, and its greater diagnostic sensitivity should help improving the quality of laboratory diagnosis, population surveys, and control of intestinal parasites.