The Impact of IFN‐γ Gene Polymorphisms on Spontaneous Clearance of HCV Infection in Fars Province, Southern of Iran. Issue 4 (20th May 2015)

• Record Type: Journal Article
• Authors: Sarvari, Jamal; Moattari, Afagh; Pirbonyeh, Neda; Moini, Maryam; Hosseini, Seyed Younes
• Is Part Of: Journal of clinical laboratory analysis. Volume 30:Issue 4(2016:Jul.)
• Page Start: 301
• Page End: 307
• Publication Date: 2015-05-20
• Subjects: HCV clearance -- hepatitis C virus -- interferon‐γ gene profile -- haplotype analysis; Diagnosis, Laboratory -- Periodicals; Medical laboratory technology -- Periodicals; 616
• DOI: 10.1002/jcla.21855
• Languages: English
• ISSNs: 0887-8013

Abstract

Abstract : Background: Certain polymorphisms in cytokine genes such as IFN‐γ may influence the outcome of hepatitis C virus (HCV) infection. Here the frequency of the genotype, allele, and haplotype of IFN‐γ gene at some loci is investigated in HCV‐infected patients. Methods: Totally 255 patients with chronic HCV infection and 44 spontaneously cleared individuals were included. The chronic or clearance states were confirmed using enzyme‐linked immunosorbent assay (ELISA) and two different qualitative reverse transcriptase polymerase chain reaction (RT‐PCR) techniques. IFN‐γ gene polymorphisms were performed by PCR using sequence‐specific primers and PCR‐RLFP on extracted genomic DNA. Results: The frequency of GG genotype ( P = 0.0001, OR: 5.69 and CI: 2.21–14.62) and allele ( P = 0.0003, OR: 2.73 and CI: 1.54–4.83) of IFN‐γ gene at +2109 locus was significantly higher in cases that spontaneously cleared the infection. Haplotype analysis showed the association of AG haplotype ( P = 0.0046, OR = 6.14 and CI = 1.56–25) with spontaneous clearance of the infection. Conclusion: Our finding indicated that individuals with GG genotype at +2109 loci of IFN‐γ gene and also AG haplotype (A allele at +874 loci and G allele at +2109 loci) may clear HCV infection more frequently than those with AA and AG genotype at +2109 loci and AA, TA, and TG haplotype.