Therapeutic deferoxamine and deferiprone monitoring in β‐thalassemia patients' plasma by field‐amplified sample injection and sweeping in capillary electrophoresis. Issue 14 (23rd May 2016)

• Record Type: Journal Article
• Authors: Lin, Hung‐Ju; Kou, Hwang‐Shang; Chiou, Shyh‐Shin; Wu, Shou‐Mei
• Is Part Of: Electrophoresis. Volume 37:Issue 14(2016)
• Page Start: 2091
• Page End: 2096
• Publication Date: 2016-05-23
• Subjects: CE -- Deferiprone -- Deferoxamine -- Stacking -- β‐Thalassemia; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201600086
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract : One CE method was established for detecting deferoxamine (DFO) and deferiprone (DFR) in plasma. For β‐thalassemia patients, DFO and DFR are major medicines to treat the iron overload caused by blood transfusion. Field‐amplified sample injection combined with sweeping was used for sensitivity enhancement in CE. This method was performed on an uncoated fused‐silica capillary. After liquid–liquid extraction, the plasma samples were electrokinetically injected into capillary at +10 kV for 180 s. The phosphate buffer (100 mM) containing 50 mM triethanolamine was used as the BGE (pH 6.6). Separation buffer was phosphate buffer (100 mM, pH 3.0) containing 150 mM SDS. This method showed good linearity ( r ≥ 0.9960). Precision and accuracy were evaluated by the results of RSD and relative error of intrabatch and interbatch analyses, and all of the absolute values were less than 6.12%. The LODs (S/N = 3) were 200 ng/mL for DFO, and 25 ng/mL for DFR. The LOQ (S/N = 10) of DFO and DFR were 600 and 75 ng/mL, respectively. This method was applied for clinical applications of five β‐thalassemia patients.