Oncostatin M overexpression induces skin inflammation but is not required in the mouse model of imiquimod‐induced psoriasis‐like inflammation. Issue 7 (12th May 2016)

• Record Type: Journal Article
• Authors: Pohin, Mathilde; Guesdon, William; Mekouo, Adela Andrine Tagne; Rabeony, Hanitriniaina; Paris, Isabelle; Atanassov, Hristo; Favot, Laure; Mcheik, Jiad; Bernard, François‐Xavier; Richards, Carl D.; Amiaud, Jérôme; Blanchard, Frédéric; Lecron, Jean‐Claude; Morel, Franck; Jégou, Jean‐François
• Is Part Of: European journal of immunology. Volume 46:Issue 7(2016)
• Page Start: 1737
• Page End: 1751
• Publication Date: 2016-05-12
• Subjects: Imiquimod · Keratinocyte · Oncostatin M · Psoriasis · Skin inflammation; Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.201546216
• Languages: English
• ISSNs: 0014-2980

Abstract

Abstract : Oncostatin M (OSM) is a cytokine locally upregulated in several skin inflammatory disorders. Here, we show that OSM exerts proinflammatory activities in vitro on mouse keratinocytes and in vivo when it is overexpressed in mouse skin. OSM induces the expression of antimicrobial peptides, chemokines, and cytokines and inhibits epidermal differentiation. Abstract : Oncostatin M (OSM) has been reported to be overexpressed in psoriasis skin lesions and to exert proinflammatory effects in vitro on human keratinocytes. Here, we report the proinflammatory role of OSM in vivo in a mouse model of skin inflammation induced by intradermal injection of murine OSM‐encoding adenovirus (AdOSM) and compare with that induced by IL‐6 injection. Here, we show that OSM potently regulates the expression of genes involved in skin inflammation and epidermal differentiation in murine primary keratinocytes. In vivo, intradermal injection of AdOSM in mouse ears provoked robust skin inflammation with epidermal thickening and keratinocyte proliferation, while minimal effect was observed after AdIL‐6 injection. OSM overexpression in the skin increased the expression of the S100A8/9 antimicrobial peptides, CXCL3, CCL2, CCL5, CCL20, and Th1/Th2 cytokines, in correlation with neutrophil and macrophage infiltration. In contrast, OSM downregulated the expression of epidermal differentiation genes, such as cytokeratin‐10 or filaggrin. Collectively, these results support the proinflammatory role of OSM when it is overexpressed in the skin. However, OSM expression was not required in the murine model of psoriasis induced by topical application of imiquimod, as demonstrated by the inflammatory phenotype of OSM‐deficient mice or wild‐type mice treated with anti‐OSM antibodies.