Frontispiece: NMR Backbone Assignment of Large Proteins by Using 13Cα‐Only Triple‐Resonance Experiments. Issue 28 (1st July 2016)

• Record Type: Journal Article
• Authors: Wei, Qingtao; Chen, Jiajing; Mi, Juan; Zhang, Jiahai; Ruan, Ke; Wu, Jihui
• Is Part Of: Chemistry. Volume 22:Issue 28(2016)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-07-01
• Subjects: backbone assignment -- covariance NMR -- isotope labeling -- NMR spectroscopy -- proteins; Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201682862
• Languages: English
• ISSNs: 0947-6539

Abstract

Abstract : Conformation Analysis NMR spectroscopy is a powerful method to investigate protein structure and dynamics, but backbone resonances in large proteins lead to difficulties. A covariance method utilizes the linear correlation coefficient between two degenerate 13 Cα peaks to accelerate assignment; a 42 kDa MDP protein backbone was assigned with 98 % accuracy. For more details, see the Full Paper by K. Ruan, J. Wu, et al. onpage 9556 ff.