Transcriptome activity of isochores during preimplantation process in human and mouse. Issue 14 (7th July 2016)

• Record Type: Journal Article
• Authors: Barton, Carl; Iliopoulos, Costas S.; Pissis, Solon P.; Arhondakis, Stilianos
• Is Part Of: FEBS letters. Volume 590:Issue 14(2016)
• Page Start: 2297
• Page End: 2306
• Publication Date: 2016-07-07
• Subjects: base composition -- expression -- GC level -- isochores -- preimplantation -- RNA‐seq -- transcriptome; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.12245
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : This work investigates the role of isochores during preimplantation process. Using RNA‐seq data from human and mouse preimplantation stages, we created the spatio‐temporal transcriptional profiles of the isochores during preimplantation. We found that from early to late stages, GC‐rich isochores increase their expression while GC‐poor ones decrease it. Network analysis revealed that modules with few coexpressed isochores are GC‐poorer than medium‐large ones, characterized by an opposite expression as preimplantation advances, decreasing and increasing respectively. Our results reveal a functional contribution of the isochores, supporting the presence of structural‐functional interactions during maturation and early‐embryonic development. Abstract : Isochores GC effects on their expression increases as preimplantation advances. GC‐rich isochores increase their expression as preimplantation advance. From early to late preimplantation stages, GC‐rich modules follow an opposite expression pattern compared with the GC‐poor ones, increasing and decreasing, respectively.