Development of rapid and highly specific TaqMan probe-based real-time PCR assay for the identification and enumeration of Lactobacillus kefiri in kefir milk. (October 2016)

• Record Type: Journal Article
• Authors: Kim, Dong-Hyeon; Kang, Il-Byeong; Jeong, Dana; Kim, Hyunsook; Kim, Hong-Seok; Lee, Soo-Kyoung; Song, Kwang-Young; Seo, Kun-Ho
• Is Part Of: International dairy journal. Volume 61(2016:Oct.)
• Page Start: 18
• Page End: 21
• Publication Date: 2016-10
• Subjects: Dairying -- Periodicals; Industrie laitière -- Périodiques; Electronic journals; 338.1762142
• DOI: 10.1016/j.idairyj.2016.03.007
• Languages: English
• ISSNs: 0958-6946

Abstract

Abstract: Lactobacillus kefiri is one of the key functional lactic acid bacteria in kefir milk. We designed a novel real-time PCR primer/probe set, LK_508 targeting the rec A gene, for the rapid identification and enumeration of L. kefiri . In inclusivity and exclusivity test using standard strains and kefir isolates, only the 9 tested L. kefiri strains were positive, with the remaining 38 closely related microorganisms testing negative, thus indicating 100% sensitivity and specificity of the assay. The population of L. kefiri was 3.77, 4.30, 4.79, and 5.63 log cfu mL −1 of kefir milk fermented at 25 °C with 5% grain-milk ratio for 12, 24, 36, and 48 h, respectively. The newly developed qPCR assay could be applied to investigate the quantitative relationship of kefir microbiota in fermentation process.