Evaluation of immunogenicity and protective efficacy of adjuvanted Salmonella Typhimurium ghost vaccine against salmonellosis in chickens. Issue 3 (2nd July 2016)

• Record Type: Journal Article
• Authors: Jawale, Chetan V.; Lee, John Hwa
• Is Part Of: Veterinary quarterly. Volume 36:Issue 3(2016)
• Page Start: 130
• Page End: 136
• Publication Date: 2016-07-02
• Subjects: Poultry -- chicken -- bacterial ghost -- Salmonella Typhimurium -- vaccine; Veterinary medicine -- Periodicals; Veterinary Medicine; Veterinary medicine -- Periodicals; Veterinary research -- Periodicals; Veterinary medicine; Periodicals; Periodicals; 636.089
• DOI: 10.1080/01652176.2016.1138248
• Languages: English
• ISSNs: 0165-2176

Abstract

ABSTRACT: Background: Salmonella Typhimurium, a non-host-adapted Gram-negative intracellular pathogen, is capable of infecting a variety of animal hosts and humans. Objective: This study utilized the prime-booster immunization strategy using Salmonella Typhimurium–LTB (S. Typhimurium–LTB) ghost with the aim of inducing a robust immune response for the prevention of avian salmonellosis. In addition, the effect of Montanide TM ISA 70VG adjuvant on S. Typhimurium–LTB ghost vaccination was investigated. Animals and methods: A total of 75 chickens were divided into three groups (n=25) for intramuscular immunization: group A (non-immunized control injected with sterile PBS), group B (immunized with S. Typhimurium–LTB ghost), and group C (immunized with S. Typhimurium–LTB ghost plus Montanide TM ISA70VG adjuvant). Results: Compared with group A, the immunized chickens (groups B and C) exhibited increased titers of antigen specific plasma IgG and intestinal secretory IgA antibodies. In addition, group C showed enhanced induction of the humoral immune response compared to group B. The populations of splenic CD3+CD4+ and CD3+CD8+ T-cells increased significantly in both immunized groups. In addition, increased mRNA expression of the Th1 cytokines, IFN-γ, and IL-2 were observed in S. Typhimurium antigen-stimulated peripheral blood mononuclear cells from groups B and C chickens. Chickens from both vaccinated groups showed significant protection against virulent S. Typhimurium oral challenge compared to non-vaccinated chickens and a lower challenge strain count was recovered from the internal organs of group C. Conclusions: Injection of S. Typhimurium-LTB ghost with or without Montanide TM ISA70VG adjuvant is capable of inducing protective immunity against the virulent S. Typhimurium infection in chickens.