BCL11A expression in acute phase chronic myeloid leukemia. (August 2016)

• Record Type: Journal Article
• Authors: Yin, Jiawei; Zhang, Fan; Tao, Huiquan; Ma, Xiao; Su, Guangsong; Xie, Xiaoli; Xu, Zhongjuan; Zheng, Yanwen; Liu, Hong; He, Chao; Mao, Zhengwei Jenny; Wang, Zhiwei; Chang, Weirong; Gale, Robert Peter; Wu, Depei; Yin, Bin
• Is Part Of: Leukemia research. Volume 47(2016:Aug.)
• Page Start: 88
• Page End: 92
• Publication Date: 2016-08
• Subjects: Leukemia -- Chronic myeloid leukemia -- BCL11A expression -- Differentiation; Leukemia -- Periodicals; Leukemia -- Periodicals; Leucémie -- Périodiques; Leukemia; Periodicals; Electronic journals; Electronic journals; 616.9941905
• DOI: 10.1016/j.leukres.2016.05.018
• Languages: English
• ISSNs: 0145-2126

Abstract

Highlights: BCL11A expression is increased in 11% chronic and 53% acute phase CML. BCL11A levels are higher in acute than chronic phase. BCL11A expression is correlated with percent bone marrow blasts. BCL11A levels are decreased in K562 cells induced to differentiate. Ectopic expression of BCL11A in K562 cells blocks differentiation. Abstract: Chronic myeloid leukemia (CML) has chronic and acute phases. In chronic phase myeloid differentiation is preserved whereas in acute phase myeloid differentiation is blocked. Acute phase CML resembles acute myeloid leukemia (AML). Chronic phase CML is caused by BCR-ABL1 . What additional mutation(s) cause transition to acute phase is unknown and may differ in different persons with CML. BCL11A encodes a transcription factor and is aberrantly-expressed in several haematological and solid neoplasms. We analyzed BCL11A mRNA levels in subjects with chronic and acute phase CML. BCL11A transcript levels were increased in subjects with CML in acute phase compared with those in normals and in subjects in chronic phase including some subjects studied in both phases. BCL11A mRNA levels were correlated with percent bone marrow blasts and significantly higher in lymphoid versus myeloid blast crisis. Differentiation of K562 with butyric acid, a CML cell line, decreased BCL11A mRNA levels. Cytology and flow cytometry analyses showed that ectopic expression of BCL11A in K562 cells blocked differentiation. These data suggest BCL11A may operate in transformation of CML from chronic to acute phase in some persons.