Protective effects of vitamin D3 against d-galactosamine-induced liver injury in rats. Issue 4 (August 2016)

• Record Type: Journal Article
• Authors: Colakoglu, Neriman; Kuloglu, Tuncay; Ozan, Enver; Kocaman, Nevin; Dabak, Durrin Ozlem; Parlak, Gozde
• Is Part Of: Tissue & cell. Volume 48:Issue 4(2016)
• Page Start: 356
• Page End: 360
• Publication Date: 2016-08
• Subjects: d-Galactosamine -- Liver injury -- Vitamin D3 -- Apoptosis -- TUNEL -- Microscope; Cytology -- Periodicals; 571.5
• DOI: 10.1016/j.tice.2016.05.003
• Languages: English
• ISSNs: 0040-8166

Abstract

Highlights: In animal modeling, d -galactosamine (d -GaIN) was injected to induce liver damage. d -GaIN caused inflamation in the liver tissue. Moreover, sinusoidal dilattion, depleted glycogen content of hepatocytes were the other results of the this experimental study. Inflamation caused byd -GaIN triggered the apoptotic process gradually in the liver. Vitamin D3 treatment provided structural integrity of the liver againstd -GaIN exposure. Abstract: In this study, we examined liver damage induced byd -galactosamine (d -GaIN) and the protective effects of vitamin D3 in relation tod -GaIN toxicity. Twenty Wistar albino rats were used in this study. The rats were divided into four groups. Group I rats were used as the control group. Group II rats were given a single intraperitoneal injection ofd -GaIN. Group III rats were given a single intraperitoneal injection ofd -GaIN, intramuscular vitamin D3 for five days. Group IV rats were given intramuscular vitamin D3 for five days. All of rats were euthanized by cervical decapitation on the fifth day of experiment. Upon completion of the experiment, a midsaggital incision was performed, and the livers of all rats were removed and fixed. The livers were processed to perform TUNEL technique and histochemical staining. During the microscope examination, we observed inflamatory cell infiltration, sinusoidal dilatation, and apoptotic bodies due tod -GaIN exposure. In addition, glycogen content of the group II hepatocytes was significantly decreased. Vitamin D3 treatment provided better structural apperance of the livers in group III. TUNEL positive cells were extremly pervasive in the group II livers. The study found group III TUNEL positive cells at a reduced rate in relation to group II due to vitamin D3 treatment. This findings indicate thatd -GaIN causes inflamation in the liver. This inflamation triggers the apoptotic process gradually. Vitamin D3 has potency to decrease the severity ofd -GaIN-caused structural liver damage.