Microbial biodegradation of proteinaceous tannery solid waste and production of a novel value added product – Metalloprotease. (October 2016)

• Record Type: Journal Article
• Authors: Ravindran, Balasubramani; Wong, Jonathan W.C.; Selvam, Ammaiyappan; Thirunavukarasu, Kathirvel; Sekaran, Ganesan
• Is Part Of: Bioresource technology. Volume 217(2016)
• Page Start: 150
• Page End: 156
• Publication Date: 2016-10
• Subjects: Clostridium limosum -- Acid protease -- Tannery solid waste -- Response surface methodology -- Fermentation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.03.033
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Utilization of proteinaceous waste for production of novel extracellular acid metalloprotease. Reduction of pollutant load through the microbial degradation of tannery solid waste. Hydrolysis of animal fleshing through protease activity in the anaerobic fermentation process. Clostridium limosum, a potential candidate for simultaneous degradation of tannery waste and production of metalloprotease. Abstract: In this study, animal fleshing (ANFL) was utilized as a substrate for the production of extracellular protease by Clostridium limosum through central composite rotatable design (CCRD) and response surface methodology (RSM). Optimum protease production of 433 U/ml was achieved and the purified enzyme was identified as acidic metalloprotease, a monomeric protein. The molecular weight of the enzyme was 71 kDa, whose activity was enhanced by bivalent metals such as Zn 2+ and Mg 2+ . Scanning electron microscopy (SEM) examination also revealed the hydrolysis/microbial degradation of ANFL through protease activity in the anaerobic fermentation process. Simultaneous hydrolysis of ANFL and production of an enzyme with the potential for different industrial applications provide an attractive methodology for the disposal of tannery solid waste.