Biotinylated N-palmitoyl chitosan for design of drug loaded self-assembled nanocarriers. (August 2016)

• Record Type: Journal Article
• Authors: Balan, Vera; Redinciuc, Valentina; Tudorachi, Nita; Verestiuc, Liliana
• Is Part Of: European polymer journal. Volume 81(2016:Aug.)
• Page Start: 284
• Page End: 294
• Publication Date: 2016-08
• Subjects: BPCs biotinylated N-palmitoyl chitosan -- CMC critical micelle concentration -- DCX Docetaxel -- DCX-NP docetaxel-loaded nanoparticles -- DL drug loading -- DLS dynamic light scattering -- DMSO dimethyl sulfoxide -- EDAC 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide hydrochloride -- EE entrapment efficiency -- GPC gel permeation cromatography -- 1H NMR Proton Nuclear Magnetic Resonance -- Mn number-average molecular weight -- Mw weight-average molecular weight -- NHS N-hydroxysuccinimide -- PCs N-palmitoyl chitosan -- PBS phosphate buffered saline -- TEM Transmission Electron Microscopy -- TNBS trinitrobenzene sulphonic acid; Biotin -- N-palmitoyl chitosan -- Self-assembled nanocarries -- Docetaxel -- Hemocompatibility; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; Polymerization; Polymers; Periodicals; Electronic journals; 547.705
• DOI: 10.1016/j.eurpolymj.2016.06.014
• Languages: English
• ISSNs: 0014-3057

Abstract

Graphical abstract: Highlights: N-palmitoyl chitosan is biofunctionalized with biotin, via carbodiimide chemistry. Biotinylated N-palmitoyl chitosan is able to self-assemble as nanocarriers. Chemotherapeutic agents are entrapped simultaneously with nanocarrier generation. Nanoparticles exhibit a pH-dependent release profile, biodegradability and hemocompatibility. Abstract: Owing to its remarkable properties, biotin has been proposed as a very active targeting molecule in anti-cancer formulations. In this study, biotinylated N-palmitoyl chitosan has been prepared and investigated as suitable polymeric derivative for preparation of docetaxel self-assembled nanocarriers with applications in breast cancer treatment. Biotinylated N-palmitoyl chitosan has been obtained through the reaction of N-palmitoyl chitosan with biotin, via carbodiimide chemistry; its structure has been confirmed by FT-IR, 1 H NMR spectroscopy and thermal analyses. The self-aggregation behaviour of the polymer in aqueous was determined using fluorescence spectroscopy. Docetaxel loaded self-assembled nanoparticles based on biotinylated N-palmitoyl chitosan, prepared by combining the dialysis method with ultrasonication, were nearly spherical in shape, with narrow size distribution, in the range of 300–400 nm and positive charged. Nanoparticles exhibited a good drug loading ability and a pH-dependent release profile of drug, susceptibility to biodegradation and hemocompatibility, properties which can be exploited in drug delivery applications.