Dual-priming oligonucleotide-based multiplex PCR using tissue samples from the rapid urease test kit for the detection of Helicobacter pylori in bleeding peptic ulcers. Issue 8 (August 2016)

• Record Type: Journal Article
• Authors: Chung, Woo Chul; Jeon, Eun Jung; Oh, Jung Hwan; Park, Jae Myung; Kim, Tae Ho; Cheung, Dae Young; Kim, Byung Wook; Kim, Sung Soo; Kim, Jin Il
• Is Part Of: Digestive and liver disease. Volume 48:Issue 8(2016)
• Page Start: 899
• Page End: 903
• Publication Date: 2016-08
• Subjects: Diagnosis -- Dual-priming oligonucleotide-based multiplex polymerase chain reaction -- Gastrointestinal bleeding -- Helicobacter pylori; Digestive organs -- Diseases -- Periodicals; Liver -- Diseases -- Periodicals; 616.33005
• DOI: 10.1016/j.dld.2016.04.012
• Languages: English
• ISSNs: 1590-8658

Abstract

Abstract: Background: In patients with peptic ulcer bleeding (PUB), diagnostic tests for Helicobacter pylori ( H. pylori ) infection have low sensitivity. The aim of our study was to investigate the diagnostic yield of dual-priming oligonucleotide-based multiplex (DPO)-PCR using tissue samples from the rapid urease test (RUT, CLO ® test) kit in patients with PUB. Methods: We prospectively enrolled patients with PUB. During second-look endoscopy, gastric biopsy specimens for histology and RUT were obtained from a total of 170 patients. DPO-PCR tests were performed on tissue samples obtained from the CLO ® test kit. If testing for H. pylori was negative, endoscopy with re-biopsy was performed 8 weeks after the bleeding episode. Results: H. pylori -associated bleeding was confirmed in 64.1% (109/170) of the patients. At the bleeding episode, the diagnostic sensitivities of RUT, histology, and DPO-PCR test were 47.7% (52/109), 71.6% (78/109) and 97.2% (106/109), respectively ( p < 0.01). The specificity of the DPO-PCR test was 91.8% (56/61). The positive predictive value (PPV) of the DPO-PCR test was 95.5% (106/111), and its negative predictive value (NPV) was 94.9% (56/59). Conclusions: In patients with PUB, the DPO-PCR test could be a useful diagnostic tool for H. pylori infection. Particularly given a negative RUT result, subsequent DPO-PCR testing of tissue samples from the CLO ® test kit could be of considerable benefit.