Histological and molecular analysis of the long-pulse 1, 064-nm Nd:YAG laser irradiation on the ultraviolet-damaged skin of hairless mice: In association with pulse duration change. (2nd January 2016)

• Record Type: Journal Article
• Authors: Rhee, Do Young; Cho, Hong Il; Park, Gyeong-Hun; Moon, Hye-Rim; Chang, Sung Eun; Won, Chong Hyun; Jung, Joon Min; Park, Ki-Young; Lee, Mi Woo; Choi, Jee Ho; Moon, Kee Chan; Lee, Deug-Chan; Goo, Boncheol
• Is Part Of: Journal of cosmetic and laser therapy. Volume 18:Number 1(2016:Feb.)
• Page Start: 16
• Page End: 21
• Publication Date: 2016-01-02
• Subjects: collagen remodeling -- Nd:YAG laser -- pulse duration -- rejuvenation -- wound healing; Skin -- Laser surgery -- Periodicals; Surgery, Plastic -- Periodicals; 617.477
• DOI: 10.3109/14764172.2015.1052509
• Languages: English
• ISSNs: 1476-4172

Abstract

Abstract : Background: Nonablative lasers have been widely used to improve photodamaged skin, although the mechanism underlying dermal collagen remodeling remains unclear.Objective: To investigate the effects and the molecular mechanisms of long-pulse neodymium-doped yttrium aluminum garnet (Nd:YAG) laser irradiation on dermal collagen remodeling in association with different pulse durations.Material and methods: Five hairless mice were pretreated with ultraviolet B irradiation for 8 weeks. The dorsal quadrant of each mouse was then irradiated twice at 1-week intervals at a pulse duration of 1 ms, 12 ms, or 50 ms, and a constant fluence of 20 J/cm 2 . The levels of dermal collagen, mRNAs of procollagens, matrix metalloproteinases (MMPs), tissue inhibitor of metalloproteinases (TIMPs), and various growth factors were analyzed after 4 weeks.Results: Long-pulse Nd:YAG treatment increased the dermal collagen level. A substantial increase in the level of procollagens, MMPs, TIMPs, and various growth factors was also observed irrespective of pulse duration, with a trend toward maximal increase at a pulse duration of 12 ms.Conclusion: Long-pulse 1, 064-nm Nd:YAG laser irradiation promotes wound-healing process, which is characterized by the induction of growth factor expression and subsequent increase in MMPs and TIMPs, followed by matrix remodeling as confirmed by new procollagen production.