Baccharis dracunculifolia methanol extract enhances glucose-stimulated insulin secretion in pancreatic islets of monosodium glutamate induced-obesity model rats. (2nd July 2016)

• Record Type: Journal Article
• Authors: Hocayen, Palloma de A. S.; Grassiolli, Sabrina; Leite, Nayara C.; Pochapski, Márcia T.; Pereira, Ricardo A.; da Silva, Luiz A.; Snack, Andre L.; Michel, R. Garcia; Kagimura, Francini Y.; da Cunha, Mário A. A.; Malfatti, Carlos R. M.
• Is Part Of: Pharmaceutical biology. Volume 54:Number 7(2016:Jul.)
• Page Start: 1263
• Page End: 1271
• Publication Date: 2016-07-02
• Subjects: β Cells -- metabolism -- phenolic compounds; Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2015.1067232
• Languages: English
• ISSNs: 1388-0209

Abstract

Abstract: Context : Obesity is the main risk factor for type 2 diabetes mellitus. Secondary metabolites with biological activities and pharmacological potential have been identified in species of the Baccharis genus that are specifically distributed in the Americas. Objective : This study evaluated the effects of methanol extracts from Baccharis dracunculifolia DC. Asteraceae on metabolic parameters, satiety, and growth in monosodium glutamate (MSG) induced-obesity model rats. Materials and methods : MSG was administered to 32 newborn rats (4 mg/g of body weight) once daily for 5 consecutive days. Four experimental groups (control, control + extract, MSG, and MSG + extract) were treated for 30 consecutive days with 400 mg/kg of B. dracunculifolia extract by gavage. Biochemical parameters, antioxidant activity, total extract phenolic content (methanolic, ethanolic, and acetone extractions), and pancreatic islets were evaluated. Results : High levels of phenolic compounds were identified in B. dracunculifolia extracts (methanol: 46.2 ± 0.4 mg GAE/L; acetate: 70.5 ± 0.5 mg GAE/L; and ethanol: 30.3 ± 0.21 mg GAE/L); high antioxidant activity was detected in B. dracunculifolia ethanol and methanol extracts. The concentration of serum insulin increased 30% in obese animals treated with extract solutions (1.4–2.0 µU/mL, p < 0.05). Insulin secretion in pancreatic islets was 8.3 mM glucose (58%, p < 0.05) and 16.7 mM (99.5%, p < 0.05) in rats in the MSG + extract and MSG groups, respectively. Discussion and conclusion : Treatment with B. dracunculifolia extracts protected pancreatic islets and prevented the irreversible cellular damage observed in animals in obesity and diabetes models.