A diarylpentanoid curcumin analog exhibits improved radioprotective potential in the intestinal mucosa. (2nd July 2016)

• Record Type: Journal Article
• Authors: Fukuda, Koji; Uehara, Yoshihiko; Nakata, Eiko; Inoue, Masahiro; Shimazu, Kazuhiro; Yoshida, Taichi; Kanda, Hiroaki; Nanjo, Hiroshi; Hosoi, Yoshio; Yamakoshi, Hiroyuki; Iwabuchi, Yoshiharu; Shibata, Hiroyuki
• Is Part Of: International journal of radiation biology. Volume 92:Number 7(2016:Jul.)
• Page Start: 388
• Page End: 394
• Publication Date: 2016-07-02
• Subjects: Curcumin -- radioprotection -- intestinal damage; Radiation -- Physiological effect -- Periodicals; Radiobiology -- Periodicals; 571.45
• DOI: 10.3109/09553002.2016.1164910
• Languages: English
• ISSNs: 0955-3002

Abstract

Abstract: Purpose : To best enhance the effects of radiotherapy, it is important to minimize adverse events, including free radical-induced intestinal cell damage. Given the threat of nuclear power plant accidents or nuclear terrorism, there is an urgent need for radioprotectants to counteract the radiation-induced toxicity and/or injuries. Curcumin exhibits protective effects against gamma irradiation; however, its in vivo efficacy is decreased due to the low bioavailability. We examined the radioprotective effect of a newly synthesized curcumin analog, GO-Y031, on 11-Gy X-ray-induced intestinal mucosal damage in mice.Materials and methods : The radioprotection experiments were conducted by using C57BL/6J or Jcl:ICR mice. Molecules related to radiation damage, including p53, Bax, Bcl-2, cleaved caspase-3, and reactive carbonyl species (RCS), were investigated immunohistochemically.Results : GO-Y031 protected against crypt hypoplasia relative to a mock treatment at 0.5% (weight/weight); the number of crypts were 11.00 ± 2.00/circumference (mm) in treated versus 6.86 ± 0.99/mm in mock-treated C57BL/6 mice ( p = 0.0079). GO-Y031 also reduced the levels of RCS, p53, and cleaved caspase-3 accumulation in the irradiated intestinal cells.Conclusions : GO-Y031 suppresses the accumulation of RCS and apoptosis-related molecules in irradiated cells. This compound may be a good primary radioprotective compound.