Assessing corneal biomechanics with Brillouin spectro-microscopy. (6th April 2016)

• Record Type: Journal Article
• Authors: Lepert, Guillaume; Gouveia, Ricardo M.; Connon, Che J.; Paterson, Carl
• Is Part Of: Faraday discussions. Volume 187(2016)
• Page Start: 415
• Page End: 428
• Publication Date: 2016-04-06
• Subjects: Chemistry -- Periodicals; Metallurgy -- Periodicals; Electrochemistry -- Periodicals; 540
• DOI: 10.1039/c5fd00152h
• Languages: English
• ISSNs: 1359-6640

Abstract

Abstract : A new Brillouin spectro-microscope was designed and built to investigate the mechanical properties of bovine and human corneas. This instrument integrates a single-stage virtually imaged phased array spectrometer with a novel adaptive-optics interferometric filter to achieve unprecedented rejection of the elastic background signal. As a result, highly-resolved, reproducible data from both thin and thick collagen-based materials were obtained. In particular, this technique is capable of rigorously measuring the relative stiffness of different areas of human corneas, thus providing a true non-contact method to characterise the fundamental mechanical features of both live and fixed biological tissue samples.