Advancements in quantum cascade laser-based infrared microscopy of aqueous media. (1st April 2016)

• Record Type: Journal Article
• Authors: Haase, K.; Kröger-Lui, N.; Pucci, A.; Schönhals, A.; Petrich, W.
• Is Part Of: Faraday discussions. Volume 187(2016)
• Page Start: 119
• Page End: 134
• Publication Date: 2016-04-01
• Subjects: Chemistry -- Periodicals; Metallurgy -- Periodicals; Electrochemistry -- Periodicals; 540
• DOI: 10.1039/c5fd00177c
• Languages: English
• ISSNs: 1359-6640

Abstract

Abstract : The large mid-infrared absorption coefficient of water frequently hampers the rapid, label-free infrared microscopy of biological objects in their natural aqueous environment. However, the high spectral power density of quantum cascade lasers is shifting this limitation such that mid-infrared absorbance images can be acquired in situ within signal-to-noise ratios of up to 100. Even at sample thicknesses well above 50 μm, signal-to-noise ratios above 10 are readily achieved. The quantum cascade laser-based microspectroscopy of aqueous media is exemplified by imaging an aqueous yeast solution and quantifying glucose consumption, ethanol generation as well as the production of carbon dioxide gas during fermentation.