Induction of HEXIM1 activities by HMBA derivative 4a1: Functional consequences and mechanism. Issue 1 (28th August 2016)

• Record Type: Journal Article
• Authors: Ketchart, Wannarasmi; Yeh, I-Ju; Zhou, Haoyan; Thiagarajan, Praveena S.; Lathia, Justin; Reizes, Ofer; Exner, Agata; Su, Bin; Montano, Monica M.
• Is Part Of: Cancer letters. Volume 379:Issue 1(2016)
• Page Start: 60
• Page End: 69
• Publication Date: 2016-08-28
• Subjects: HMBA -- HEXIM1 -- Breast cells -- Differentiation -- Antiestrogens -- Metastasis; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2016.05.029
• Languages: English
• ISSNs: 0304-3835

Abstract

Highlights: 4a1 induced breast cell differentiation. 4a1 enhanced inhibitory effects of tamoxifen. 4a1 inhibited breast tumor metastasis. Mediators of the phenotypic effects of 4a1 are identified. 4a1 has a potential as lead compound for further drug development. Abstract: We have been studying the role of Hexamethylene bisacetamide (HMBA) Induced Protein 1 (HEXIM1) as a tumor suppressor whose expression is decreased in tamoxifen resistant and metastatic breast cancer. HMBA was considered the most potent and specific inducer for HMBA inducible protein 1 (HEXIM1) prior to our studies. Moreover, the ability of HMBA to induce differentiation is advantageous for its therapeutic use when compared to cytotoxic agents. However, HMBA induced HEXIM1 expression required at mM concentrations and induced dose limiting toxicity, thrombocytopenia. Thus we structurally optimized HMBA and identified a more potent inducer of HEXIM1 expression, 4a1. The studies reported herein tested the ability of 4a1 to induce HEXIM1 activities using a combination of biochemical, cell phenotypic, and in vivo assays. 4a1 induced breast cell differentiation, including the stem cell fraction in triple negative breast cancer cells. Clinically relevant HEXIM1 activities that are also induced by 4a1 include enhancement of the inhibitory effects of tamoxifen and inhibition of breast tumor metastasis. We also provide mechanistic basis for the phenotypic effects of 4a1. Our results support the potential of an unsymmetrical HMBA derivative, such as 4a1, as lead compound for further drug development.