Population structure and antimicrobial profile of Staphylococcus aureus strains associated with bovine mastitis in China. (August 2016)

• Record Type: Journal Article
• Authors: Zhang, Lili; Li, Yuchen; Bao, Hongduo; Wei, Ruicheng; Zhou, Yan; Zhang, Hui; Wang, Ran
• Is Part Of: Microbial pathogenesis. Volume 97(2016)
• Page Start: 103
• Page End: 109
• Publication Date: 2016-08
• Subjects: Staphylococcus aureus -- Bovine mastitis -- MLST typing -- Biofilm production -- Invasion assay; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2016.06.005
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Staphylococcus aureus is a significant bacterial pathogen associated with bovine mastitis. The aim of the present study was to investigate and characterize of S. aureus strains isolated from the milk of cows suffering from mastitis in the mid-east of China. Among the 200 milk samples analyzed, 58 were positive for S. aureus, of these isolates, 11 isolates were methicillin-resistant Staphylococcus aureus (MRSA). All of the 58 S. aureus strains were classified in agr group I, while seven different sequence type (ST) patterns were identified and among them the most common was ST630 followed by ST188. All of the S. aureus isolates belonging to ST630 were resistant to more than four antimicrobials, and 22.2% of isolates belonging to ST188 were resistant to eight antimicrobials. Interestingly, while strong biofilm producers demonstrated higher resistance to multiple antimicrobials, they exhibited lower intracellular survival rates. The results of this study illustrated the distribution, antimicrobial susceptibility profiles, genotype, and the ability of biofilm production and mammary epithelial cells invasion of these S. aureus isolates. This study can provide the basis for the development of a disease prevention program in dairy farms to reduce the potential risk in both animal and human health. Highlights: The 58 (29.0%) Staphylococcus aureus strains causing bovine mastitis were isolated from Jiangsu of China, and 19.0% was MRSA. All of the 58 S. aureus isolates were classified in agr group I. ST630 and ST188 were the prominent STs in this study. Approximately 22.4% of isolates was resistant to ≥5 antimicrobials. Most strains showed a low invasion rate in MAC-T cells and formed a strong biofilm in TSBglc .