Transcription of exsD is repressed directly by H-NS in Vibrio parahaemolyticus. (August 2016)

• Record Type: Journal Article
• Authors: Zhang, Yiquan; Osei-Adjei, George; Ni, Bin; Fang, Haihong; Zhang, Lingyu; Zhao, Xin; Huang, Xinxiang; Yang, Huiying; Yang, Wenhui; Sun, Fengjun
• Is Part Of: Microbial pathogenesis. Volume 97(2016)
• Page Start: 221
• Page End: 225
• Publication Date: 2016-08
• Subjects: Vibrio parahaemolyticus -- T3SS1 -- exsD -- H-NS; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2016.06.003
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Vibrio parahaemolyticus is a leading cause of seafood-associated diarrhea and gastroenteritis. This bacteria expresses a major virulence determinant called T3SS1. Expression of T3SS1 is tightly regulated by the ExsA-ExsC-ExsD regulatory system. The transcription of exsA and probably exsC is repressed directly by the H-NS protein. In this study, the regulation of exsD by H-NS was investigated using quantitative RT-PCR, primer extension, LacZ fusion, electrophoretic mobility shift, and DNase I footprinting assays. The results showed that His-H-NS protected a single region from 61 bp to 174 bp upstream of exsD against DNase I digestion, and a transcription start site located at 105 bp upstream of exsD was detected and its activity was repressed by H-NS. Therefore, a single H-NS-dependent promoter was transcribed for exsD in V. parahaemolyticus . Thus, all three genes in the ExsA-ExsC-ExsD regulatory system of T3SS1 are directly repressed by H-NS in V. parahaemolyticus .