Broadening substrate specificity of a chain-extending ketosynthase through a single active-site mutation. Issue 54 (16th June 2016)

• Record Type: Journal Article
• Authors: Murphy, Annabel C.; Hong, Hui; Vance, Steve; Broadhurst, R. William; Leadlay, Peter F.
• Is Part Of: Chemical communications. Volume 52:Issue 54(2016)
• Page Start: 8373
• Page End: 8376
• Publication Date: 2016-06-16
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c6cc03501a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : An in vitro model system based on a ketosynthase domain of the erythromycin polyketide synthase was used to probe the apparent substrate tolerance of ketosynthase domains of the mycolactone polyketide synthase. Abstract : An in vitro model system based on a ketosynthase domain of the erythromycin polyketide synthase was used to probe the apparent substrate tolerance of ketosynthase domains of the mycolactone polyketide synthase. A specific residue change was identified that led to an emphatic increase in turnover of a range of substrates.