A "turn-on" fluorescent probe for the detection of Cu2+ in living cells based on a signaling mechanism of NN isomerization. (17th May 2016)

• Record Type: Journal Article
• Authors: Cao, Shuo; Jin, Qiaoying; Geng, Lin; Mu, Lingyun; Dong, Shouliang
• Is Part Of: New journal of chemistry. Volume 40:Number 7(2016:Jul.)
• Page Start: 6264
• Page End: 6269
• Publication Date: 2016-05-17
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c5nj03649f
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : A "turn-on" probeA based on coumarin with a mechanism of NN isomerization was developed for detecting Cu 2+ in living cells. Abstract : A novel fluorescent probeA based on coumarin associated with a NN double bond was conveniently designed and synthesized for Cu 2+, and both rapid colorimetric and fluorescence turn-on detection can be achieved, showing a visible color change from pink to yellow and a significant fluorescence enhancement, respectively. The probe exhibits high selectivity toward Cu 2+ over other metal ions and excellent sensitivity with a detection limit of 20 nM, and it can be monitored in a wide range of pH (3–10) in aqueous systems. Besides, the probe has been applied in living cells.