DNA structure-specific sensitization of a metalloporphyrin leads to an efficient in vitro quadruplex detection molecular tool. (16th May 2016)

• Record Type: Journal Article
• Authors: Diabate, Pape Diaba; Laguerre, Aurélien; Pirrotta, Marc; Desbois, Nicolas; Boudon, Julien; Gros, Claude P.; Monchaud, David
• Is Part Of: New journal of chemistry. Volume 40:Number 7(2016:Jul.)
• Page Start: 5683
• Page End: 5689
• Publication Date: 2016-05-16
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c6nj01012a
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : The palladated porphyrin Pd·TEGPy is an efficient turn-on, quadruplex-selective fluorophore thanks to peculiar supramolecular and electronic properties that results in a unique DNA structure-specific sensitization mechanism. Abstract : The search for convenient molecular probes for detecting DNA and RNA quadruplexes in vitro is marked by a rapid pace of progress, spurred on by the multiple roles these higher-order nucleic acid structures play in many genetic dysregulations. Here, we contribute to this search, reporting on a palladated porphyrin named Pd·TEGPy: its efficiency as quadruplex-selective fluorescent dye relies on a structural design that endows it with attractive supramolecular and electronic properties and makes it an efficient turn-on, quadruplex-selective fluorescent stain thanks to a DNA-mediated sensitization mechanism that ensures a high level of specificity.