Novel anthracene- and pyridine-containing Schiff base probe for selective "off–on" fluorescent determination of Cu2+ ions towards live cell application. (11th May 2016)

• Record Type: Journal Article
• Authors: Simon, Turibius; Shellaiah, Muthaiah; Srinivasadesikan, Venkatesan; Lin, Ching-Chang; Ko, Fu-Hsiang; Sun, Kien Wen; Lin, Ming-Chang
• Is Part Of: New journal of chemistry. Volume 40:Number 7(2016:Jul.)
• Page Start: 6101
• Page End: 6108
• Publication Date: 2016-05-11
• Subjects: Chemistry -- Periodicals; Chimie -- Périodiques; 540
• DOI: 10.1039/c5nj03168k
• Languages: English
• ISSNs: 1144-0546

Abstract

Abstract : A simple anthracene-basedAP probe was synthesized to detect Cu 2+ ions, via the photoinduced electron transfer mechanism, in live cells. Abstract : The novel anthracene- and pyridine-containing Schiff base derivative 2-(2-(anthracen-9-ylmethylene)hydrazinyl)pyridine (AP ) has been synthesized via one-pot reaction and its fluorescent "off–on" detection of Cu 2+ ions, via PET-based mechanism, is reported. The 1 : 1 stoichiometry ofAP ⋯Cu 2+ sensor complex was calculated from Job's plot based on PL titrations and supported by ESI (+Ve) mass spectral analysis. In addition, the binding sites ofAP ⋯Cu 2+ sensor conjugate were supported by 1 H NMR titration. The detection limit (LOD) and association constant ( K a ) ofAP ⋯Cu 2+ complex were obtained by standard deviation and linear fittings. Furthermore, quantum yield ( Φ f ), SEM analysis, pH effect, FTIR interpretation and density functional theory (DFT) studies were conducted for theAP ⋯Cu 2+ sensing conjugate. More decisively, confocal fluorescence microscopy imaging from Raw264.7 cells indicated thatAP could be used as an effective fluorescent probe for the analysis of Cu 2+ ions in living cells.