Encapsulation of FRET-based glucose and maltose biosensors to develop functionalized silica nanoparticles. Issue 13 (26th January 2016)

• Record Type: Journal Article
• Authors: Faccio, G.; Bannwarth, M. B.; Schulenburg, C.; Steffen, V.; Jankowska, D.; Pohl, M.; Rossi, R. M.; Maniura-Weber, K.; Boesel, L. F.; Richter, M.
• Is Part Of: Analyst. Volume 141:Issue 13(2016)
• Page Start: 3982
• Page End: 3984
• Publication Date: 2016-01-26
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c5an02573g
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Abstract : Silicate nanoparticles with immobilized FRET-based biosensors were developed for the detection of glucose and maltose. Immobilization of the protein biosensor in the nanoparticle was achieved through specific interaction between the hexa-histidine tag of the protein and a calcium–silicate complex of the silica matrix. Encapsulation of the biosensors preserved the affinity for the respective sugar. Compared to the free biosensors, encapsulation had a stabilizing effect on the biosensor towards chemical and thermal denaturation. The demonstrated immobilization strategy for specific sensing proteins paves the way towards the development of protein-inorganic nanostructures for application in metabolite analyses.