Intracellular calcium signaling regulates glomerular filtration barrier permeability: the role of the PKGIα‐dependent pathway. Issue 12 (11th June 2016)

• Record Type: Journal Article
• Authors: Piwkowska, Agnieszka; Rogacka, Dorota; Audzeyenka, Irena; Kasztan, Małgorzata; Angielski, Stefan; Jankowski, Maciej
• Is Part Of: FEBS letters. Volume 590:Issue 12(2016)
• Page Start: 1739
• Page End: 1748
• Publication Date: 2016-06-11
• Subjects: calcium -- contraction apparatus -- Insulin -- filtration barrier permeability -- podocyte; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.12228
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Podocytes are dynamic polarized cells that lie on the surface of glomerular capillaries and comprise an essential component of the glomerular filtration barrier. Insulin provoked a sustained, approximately 70%, increase in intracellular calcium concentration in podocytes. RT‐PCR revealed the presence of mRNA encoding sarco/endoplasmic reticulum calcium ATPase isoforms 1–3, and plasma membrane Ca 2+ pump (PMCA) isoforms 1, 3, 4; mRNA levels were depressed by the addition of insulin. Inhibitors of PMCA, and the Na + ‐Ca 2+ exchanger, increased podocyte permeability to albumin, induced dimerization of protein kinase G type I alpha (PKGIα), and activation of PKGIα‐dependent signaling. These data suggest the involvement of calcium and PKGIα signaling in insulin‐enhanced filtration barrier permeability in podocytes. Abstract :