Microfluidics: The future of microdissection TESE?. (3rd May 2016)

• Record Type: Journal Article
• Authors: Samuel, Raheel; Badamjav, Odgerel; Murphy, Kristin E.; Patel, Darshan P.; Son, Jiyoung; Gale, Bruce K.; Carrell, Douglas T.; Hotaling, James M.
• Is Part Of: Systems biology in reproductive medicine. Volume 62:Number 3(2016:Jun.)
• Page Start: 161
• Page End: 170
• Publication Date: 2016-05-03
• Subjects: IVF -- microfluidics -- microTESE -- sperm separation; Systems biology -- Periodicals; Andrology -- Periodicals; Generative organs, Male -- Diseases -- Periodicals; Biological systems -- Periodicals; Reproductive health -- Periodicals; Human reproduction -- Periodicals; 612.61
• DOI: 10.3109/19396368.2016.1159748
• Languages: English
• ISSNs: 1939-6368

Abstract

ABSTRACT: Non-obstructive azoospermia (NOA) is a severe form of infertility accounting for 10% of infertile men. Microdissection testicular sperm extraction (microTESE) includes a set of clinical protocols from which viable sperm are collected from patients (suffering from NOA), for intracytoplasmic sperm injection (ICSI). Clinical protocols associated with the processing of a microTESE sample are inefficient and significantly reduce the success of obtaining a viable sperm population. In this review we highlight the sources of these inefficiencies and how these sources can possibly be removed by microfluidic technology and single-cell Raman spectroscopy.