Selection of pretreatment method for enriching hydrogen-producing bacteria using anaerobic sewage sludge with three different substrates. Issue 2 (3rd March 2016)

• Record Type: Journal Article
• Authors: Chaitanya, N.; Sivaramakrishna, D.; kumar, B. Satish; Himabindu, V.; Lakshminarasu, M.; Vishwanadham, M.
• Is Part Of: Biofuels. Volume 7:Issue 2(2016)
• Page Start: 163
• Page End: 171
• Publication Date: 2016-03-03
• Subjects: biohydrogen -- pretreatment -- dark fermentation -- mixed culture -- three different substrates; Biomass energy -- Periodicals; 662.8805
• DOI: 10.1080/17597269.2015.1123969
• Languages: English
• ISSNs: 1759-7269

Abstract

ABSTRACT: The pretreatment of anaerobic sewage sludge by different methods (acid, base, Ethyline Diamine Tetra Acetic Acid (EDTA), chloroform, ammonia, heat-shock, aeration, microwave oven and chloroform) were conducted in batch tests to evaluate and compare their suitability in the enrichment of hydrogen-producing bacteria from three different carbon sources (glucose, sucrose and starch). Among the pretreatment methods, heat-shock and alkali show the higher hydrogen production at 6.5 initial pH and 35°C using 5 g/l substrate concentration. The combination of heat and alkali pre-treatment found to be the maximal hydrogen content of 71%, hydrogen production potential of 310 ml/l (1.73 mol/kg COD[chemical oxygen demand]), hydrogen production rate of 51.7 ml/h/l, hydrogen yield of 2.27mol/mol glucose and substrate degradation efficiency of 70%.