Cadaveric cardiosphere-derived cells can maintain regenerative capacity and improve the heart function of cardiomyopathy. Issue 9 (2nd May 2016)

• Record Type: Journal Article
• Authors: Sun, Yong; Chi, Di; Tan, Miaoxin; Kang, Kai; Zhang, Maomao; Jin, Xiangyuan; Leng, Xiaoping; Cao, Rui; Liu, Xianglan; Yu, Bo; Wu, Jian
• Is Part Of: Cell cycle. Volume 15:Issue 9(2016)
• Page Start: 1248
• Page End: 1256
• Publication Date: 2016-05-02
• Subjects: allograft transplantation -- cadaver -- cardiosphere-derived cells -- dilated cardiomyopathy; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2016.1160973
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Objective : Cardiosphere-derived cells (CDCs) improve cardiac function and attenuate remodeling in ischemic and non-ischemic cardiomyopathy, and are currently obtained through myocardial biopsy. However, there is not any study on whether functional CDCs may be obtained through cadaveric autopsy with similar benefits in non-ischemic cardiomyopathy.Methods : Cardiac tissues from human or mouse cadavers were harvested, plated at 4°C, and removed at varying time points to culture human CDCs (CLH-EDCs) and mouse CDCs (CM-CDCs). The differentiation and paracrine effects of CDCs were also assessed. Furthermore, intramyocardial injection of cadaveric CM-CDCs was performed in an induced dilated cardiomyopathy (DCM) model.Results : With the extension of post mortem hours, the number of CLH-EDCs and CM-CDCs harvested from autopsy specimens decreased. The expressions of von Willebrand factor (VWF) and smooth muscle actin (SMA) on CDCs were gradually reduced, however, cardiac troponin I (TNI) expression increased in the 24 h group compared to the 0 h group. CLH-EDCs were also found to have similar paracrine function in the 24 h group compared to 0 h group. 8 weeks after CM-CDCs transplantion to the injured heart, mean left ventricular ejection fraction increased in both 0 h (64.99 ± 3.4%) and 24 h (62.99 ± 2.8%) CM-CDCs-treated groups as compared to the PBS treated group (53.64 ± 5.6 cm), with a decrease in left ventricular internal diastolic diameter (0.29 ± 0.08 cm and 0.32 ± 0.04 cm in 0 h and 24 h groups, vs. 0.41 ± 0.05 cm in PBS group).Conclusion : CDCs from cadaveric autopsy are highly proliferative and differentiative, and may be used as a source for allograft transplantation, in order to decrease myocardial fibrosis, attenuate left ventricular remodeling, and improve heart function in doxorubicin-induced non-ischemic cardiomyopathy.