E-Cadherin repression increases amount of cancer stem cells in human A549 lung adenocarcinoma and stimulates tumor growth. Issue 8 (17th April 2016)

• Record Type: Journal Article
• Authors: Farmakovskaya, M.; Khromova, N.; Rybko, V.; Dugina, V.; Kopnin, B.; Kopnin, P.
• Is Part Of: Cell cycle. Volume 15:Issue 8(2016)
• Page Start: 1084
• Page End: 1092
• Publication Date: 2016-04-17
• Subjects: B-catenin -- cancer stem cells (CSCs) -- E-cadherin -- tumor progression -- wnt-signaling; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2016.1156268
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Here we show that cancer stem cells amount in human lung adenocarcinoma cell line A549 depends on E-cadherin expression. In fact, downregulation of E-cadherin expression enhanced expression of pluripotent genes (c- MYC, NESTIN, OCT3/4 and SOX2 ) and enriched cell population with the cells possessing the properties of so-called 'cancer stem cells' via activation of Wnt/β-catenin signaling. Repression of E-cadherin also stimulated cell proliferation and migration in vitro, decreased cell amount essential for xenografts formation in nude mice, increased tumors vascularization and growth. On the other hand, E-cadherin upregulation caused opposite effects i.e. diminished the number of cancer stem cells, decreased xenograft vascularization and decelerated tumor growth. Therefore, agents restoring E-cadherin expression may be useful in anticancer therapy.