A new semiautomatic device with horizontal developing chamber for gradient thin-layer chromatography. Issue 5 (2nd April 2016)

• Record Type: Journal Article
• Authors: Hałka-Grysińska, Aneta; Dzido, Tadeusz H.; Sitarczyk, Ewelina; Klimek-Turek, Anna; Chomicki, Adam
• Is Part Of: Journal of liquid chromatography & related technologies. Volume 39:Issue 5/6(2016)
• Page Start: 257
• Page End: 263
• Publication Date: 2016-04-02
• Subjects: Device for gradient TLC/HPTLC -- gradient elution -- planar chromatography -- reversed-phase system -- TLC/HPTLC; Liquid chromatography -- Periodicals; Chromatographic analysis -- Periodicals; Separation (Technology) -- Periodicals; Biotechnology -- Periodicals; Chromatography, Liquid -- Periodicals; 543.8405
• DOI: 10.1080/10826076.2016.1163177
• Languages: English
• ISSNs: 1082-6076

Abstract

ABSTRACT: A new semiautomatic device with two types of horizontal developing chamber for gradient thin-layer chromatography (TLC) that does not require special procedure of chromatographic plate preparation was developed. The new device was applied for separation of test mixture of 10 dyes showing general elution problem. In addition, a general equation for determination of relative position, , of solutes chromatographed under conditions of stepwise gradient elution with one void volume of mobile phase in TLC has been applied. A satisfactory agreement between calculated (by computer program) and experimental values of has been obtained. In the article, based on obtained results, the advantages and disadvantages of different devices for gradient elution in TLC are discussed.