In vitro anti-denaturation and anti-hyaluronidase activities of extracts and galactolipids from leaves of Impatiens parviflora DC. Issue 10 (18th May 2016)

• Record Type: Journal Article
• Authors: Grabowska, Karolina; Podolak, Irma; Galanty, Agnieszka; Załuski, Daniel; Makowska-Wąs, Justyna; Sobolewska, Danuta; Janeczko, Zbigniew; Żmudzki, Paweł
• Is Part Of: Natural product research. Volume 30:Issue 10(2016)
• Page Start: 1219
• Page End: 1223
• Publication Date: 2016-05-18
• Subjects: Impatiens parviflora -- anti-denaturation -- anti-hyaluronidase -- anti-arthritic -- (MGDG) monogalactosyldiacylglicerol -- (DGDG) digalactosyldiacylglicerol; Natural products -- Periodicals; Natural products -- Research -- Periodicals; Biological Factors -- Periodicals; Biological Products -- Periodicals; 547
• DOI: 10.1080/14786419.2015.1049175
• Languages: English
• ISSNs: 1478-6419

Abstract

Abstract : The in vitro anti-denaturation and anti-hyaluronidase activities of Impatiens parviflora extracts and isolated galactolipids (MGDG-1, DGDG-1) were investigated. This is the first report on these compounds in I. parviflora . All extracts showed anti-hyaluronidase activity, but only methanolic extract from fresh leaves exhibited significant activity against heat-induced denaturation of BSA in a dose-dependent manner. At 500 μg/mL, the extract and the reference drug showed 79.05% and 99.81% inhibition of protein denaturation, respectively. These results indicate that fresh leaves of I. parviflora may be beneficial in inflammatory conditions, especially those associated with protein denaturation, such as rheumatoid arthritis. The study revealed that only MGDG-1 showed weak activity in anti-denaturation assay but both galactolipids were potent inhibitors of hyaluronidase. MGDG-1 completely inhibited the enzyme activity at the concentration of 127.9 μg/mL. These results indicate the potential of galactolipids in the treatment of diseases associated with the loss of hyaluronic acid. Abstract :