Cytogenetic characterization and nuclear DNA content of three North African endemic Centaurea species. (3rd May 2016)

• Record Type: Journal Article
• Authors: Benamara-Bellagha, M.; Baziz, K.; Pustahija, F.; Khalfallah, N.; Siljak-Yakovlev, S.
• Is Part Of: Plant biosystems. Volume 150:Number 3(2016)
• Page Start: 501
• Page End: 511
• Publication Date: 2016-05-03
• Subjects: Fluorescent in situ hybridization -- fluorochrome banding -- genome size -- heterochromatin -- karyotype -- 5S and 35S rRNA genes; Botany -- Periodicals; Biological systems -- Periodicals; Plants -- Periodicals; 581
• DOI: 10.1080/11263504.2014.981234
• Languages: English
• ISSNs: 1126-3504

Abstract

Abstract : Three endemic Centaurea species from North Africa are investigated for the first time by chromomycin fluorochrome banding for GC-rich DNA distribution, fluorescence in situ hybridization for physical mapping of rRNA genes, and flow cytometry for genome-size assessment. Investigated species belong to three different sections and possess three basic chromosome numbers: C. tougourensis subsp . tougourensis 2 n = 4 x = 36 ( x = 9), C. musimonum 2 n = 2 x = 20 ( x = 10), and C. maroccana 2 n = 2 x = 24 ( x = 12). The number and distribution of chromomycin positive bands (CMA + ) and 18S-5.8S-26S (35S) rDNA loci were different among investigated species and ranged from 6 to 80 chromomycin bands and from 2 to 6 35S rDNA loci. The three species have just one 5S rDNA locus at intercalary position on a separate chromosome pairs, except in the case of C. musimonum in which both rDNA loci were localized on the same chromosome. All rDNA loci were co-localized with CMA + bands, except three 35S in C. musimonum . Genome size ranged from 2C = 1.66 to 2C = 2.86 pg in diploid species ( C. musimonum and C. maroccana, respectively) and to 2C = 4.51 pg in tetraploid C. tougourensis subsp. tougourensis .