Simultaneous quantitative analysis of main components in linderae reflexae radix with one single marker. Issue 8 (8th May 2016)

• Record Type: Journal Article
• Authors: Wang, Li-li; Zhang, Yun-bin; Sun, Xiao-ya; Chen, Sui-qing
• Is Part Of: Journal of liquid chromatography & related technologies. Volume 39:Issue 8(2016)
• Page Start: 422
• Page End: 427
• Publication Date: 2016-05-08
• Subjects: Linderae Reflexae Radix -- pinostrobin -- Quantitative analysis multi-components by single marker; Liquid chromatography -- Periodicals; Chromatographic analysis -- Periodicals; Separation (Technology) -- Periodicals; Biotechnology -- Periodicals; Chromatography, Liquid -- Periodicals; 543.8405
• DOI: 10.1080/10826076.2016.1169429
• Languages: English
• ISSNs: 1082-6076

Abstract

ABSTRACT: Establish a quantitative analysis of multi-components by the single marker (QAMS) method for quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four main components in Linderae Reflexae Radix. Four main components of pinostrobin, pinosylvin, pinocembrin, and 3, 5-dihydroxy-2-(1- p -mentheneyl)- trans -stilbene were selected as analytes to evaluate the quality by RP-HPLC coupled with a UV-detector. The method was evaluated by a comparison of the quantitative results between the external standard method and QAMS with a different HPLC system. The results showed that no significant differences were found in the quantitative results of the four contents of Linderae Reflexae Radix determined by the external standard method and QAMS (RSD <3%). The contents of four analytes (pinosylvin, pinocembrin, pinostrobin, and Reflexanbene I) in Linderae Reflexae Radix were determined by the single marker of pinosylvin. This fingerprint was the spectra determined by Shimadzu LC-20AT and Waters e2695 HPLC that were equipped with three different columns. GRAPHICAL ABSTRACT: