Simplified process for preparation of schizophyllan solutions for biomaterial applications. Issue 3 (2nd April 2016)

• Record Type: Journal Article
• Authors: Sutivisedsak, Nongnuch; Leathers, Timothy D.; Biresaw, Girma; Nunnally, Melinda S.; Bischoff, Kenneth M.
• Is Part Of: Preparative biochemistry & biotechnology. Volume 46:Issue 3(2016)
• Page Start: 313
• Page End: 319
• Publication Date: 2016-04-02
• Subjects: Coefficient of friction -- distiller's dried grains with solubles -- downstream processing -- schizophyllan -- surface tension -- viscosity; Biochemistry -- Technique -- Periodicals; Biotechnology -- Technique -- Periodicals; Biochemistry -- methods -- Periodicals; Biotechnology -- methods -- Periodicals; 660.6
• DOI: 10.1080/10826068.2015.1031392
• Languages: English
• ISSNs: 1082-6068

Abstract

ABSTRACT: Schizophyllan is a biopolymer commercially produced for pharmaceutical and cosmetics uses. However, schizophyllan also has potential biomaterial applications. Schizophyllan is conventionally produced from glucose and recovered by diafiltration and ultrafiltration to produce a highly purified product. Here we demonstrate a simplified process for preparation of schizophyllan solutions for biomaterial applications. Schizophyllan was produced in 1.5-L bioreactors from distiller's dried grains with solubles (DDGS), an abundant coproduct of dry grind fuel ethanol production. Downstream processing eliminated filtration and concentration steps, providing solutions containing 4.2 ± 0.3 g schizophyllan/L. Solutions contained high-molecular-weight schizophyllan and exhibited viscosity properties similar to those of commercial schizophyllan. Schizophyllan solutions showed promise as a component of biolubricants in friction and wear tests and by dynamic surface and interfacial tension measurements.