A new blue-pigmented hasleoid diatom, Haslea provincialis, from the Mediterranean Sea. Issue 2 (2nd April 2016)

• Record Type: Journal Article
• Authors: Gastineau, Romain; Hansen, Gert; Davidovich, Nikolaï A.; Davidovich, Olga; Bardeau, Jean-François; Kaczmarska, Irena; Ehrman, James M.; Leignel, Vincent; Hardivillier, Yann; Jacquette, Boris; Poulin, Michel; Morançais, Michèle; Fleurence, Joël; Mouget, Jean-Luc
• Is Part Of: European journal of phycology. Volume 51:Issue 2(2016)
• Page Start: 156
• Page End: 170
• Publication Date: 2016-04-02
• Subjects: Bacillariophyta -- cox1 -- Haslea -- marennine-like pigment -- Mediterranean Sea -- micro-Raman spectroscopy -- new species -- rbcL -- taxonomy; Algology -- Periodicals; Algae -- Periodicals; 579.8
• DOI: 10.1080/09670262.2015.1110861
• Languages: English
• ISSNs: 0967-0262

Abstract

Abstract: Haslea provincialis Gastineau, Hansen & Mouget, sp. nov., is a new, morphologically semicryptic blue diatom discovered on the French shores of the Mediterranean Sea. Like H. ostrearia and H. karadagensis, H. provinciali s shares the capacity to synthesize a marennine-like blue pigment. Sexual reproduction between clones of H. provincialis has been repeatedly observed and resulted in viable initial cells. There were no sexual interactions with sexually competent clones of H. ostrearia or H. karadagensis, as would be expected for a separate biological species. There are strong similarities between the H. provincialis pigment and the marennine produced by H. ostrearia, evidenced by UV-visible spectrophotometry and Raman spectrometry. However, unlike the marennine from H. ostrearia, no differences were found between the extracellular and the intracellular forms of the pigment in H. provincialis . This indicates that the synthesis pathways and excretion mechanisms among the three 'blue' Haslea may be species-specific. Molecular taxonomy and phylogeny (based on rbc L, cox 1 and SSU V4 DNA sequences) confirmed the distinct position of this species among the blue Haslea species. Haslea provincialis occurs in environments from which H. ostrearia has already been reported (mostly based on the presence of the blue cell vacuoles). Possible species misidentifications and the impact of the complex geological history of the Mediterranean Sea on blue diatom diversification are also discussed.