Characterization of oil-palm trunk residue degradation enzymes derived from the isolated fungus, Penicillium rolfsii c3-2(1) IBRL. Issue 12 (17th June 2016)

• Record Type: Journal Article
• Authors: Lee, Kok Chang; Arai, Takamitsu; Ibrahim, Darah; Deng, Lan; Murata, Yoshinori; Mori, Yutaka; Kosugi, Akihiko
• Is Part Of: Environmental technology. Volume 37:Issue 12(2016)
• Page Start: 1550
• Page End: 1558
• Publication Date: 2016-06-17
• Subjects: Penicillium rolfsii c3-2(1) IBRL -- oil-palm trunk residues -- lignocellulosic biomass -- lignin -- commercial enzymes; Environmental engineering -- Periodicals; Environmental protection -- Periodicals; 628.05
• DOI: 10.1080/09593330.2015.1120786
• Languages: English
• ISSNs: 0959-3330

Abstract

ABSTRACT: This study characterizes crude enzymes derived from Penicillium rolfsii c3-2(1) IBRL, a mesophilic fungus isolated from the local soil of Malaysia. Prior to enzyme activity evaluation, P. rolfsii c3-2(1) IBRL was inoculated into a broth medium containing oil-palm trunk residues for the preparation of crude enzymes. Oil-palm trunk residues were optimally hydrolysed at pH5.0 and 50°C. P. rolfsii c3-2(1) IBRL-derived crude enzymes displayed higher thermal stability compared with the commercial enzymes, Celluclast 1.5 L and Acellerase 1500. Moreover, the hydrolysing activities of the P. rolfsii c3-2(1) IBRL-derived crude enzymes (xylan, arabinan, and laminarin) were superior compared to that of Celluclast 1.5 L and Acellerase 1500, and exhibit 2- to 3-fold and 3- to 4-fold higher oil-palm trunk residues-hydrolysing specific activity, respectively. This higher hydrolysis efficiency may be attributed to the weak 'lignin-binding' ability of the P. rolfsii c3-2(1) IBRL-derived enzymes compared to the commercial enzymes.