Impact of β-glucan on the Fecal Water Genotoxicity of Polypectomized Patients. (18th May 2016)

• Record Type: Journal Article
• Authors: Turunen, Katja T.; Pletsa, Vasiliki; Georgiadis, Panagiotis; Triantafillidis, John K.; Karamanolis, Dimitrios; Kyriacou, Adamantini
• Is Part Of: Nutrition and cancer. Volume 68:Number 4(2016)
• Page Start: 560
• Page End: 567
• Publication Date: 2016-05-18
• Subjects: Cancer -- Periodicals; Nutrition -- Periodicals; Cancer; Nutrition; Periodicals; 616.9940654
• DOI: 10.1080/01635581.2016.1156713
• Languages: English
• ISSNs: 0163-5581

Abstract

ABSTRACT: The aim of the study was to determine the effect of β-glucan on the cytotoxicity and genotoxicity of polypectomized patient's fecal water (FW). Polypectomized volunteers ( n = 69) were randomly assigned to consume bread with or without β-glucan, for 3 months. FW was collected at the beginning ( t = 0), the 30th and 90th day and 2 wk after the intervention. Cytotoxicity and genotoxicity were estimated on Caco-2 cells, using trypan blue exclusion test and comet assay, respectively. Gastrointestinal symptoms were recorded and subjects kept a 3-day food diary at baseline and after completion. Trypan blue exclusion test revealed cell survival of approximately 87% after incubation with FW. The FW samples showed 49% genotoxicity at the baseline. Genotoxicity in the intervention group decreased during the trial reaching statistical significance on the 90th day compared to control. An increase was noticed 2 wk after the trial, but it still remained significantly lower compared to control. Group-specific analysis for β-glucan also revealed significant decrease in the genotoxicity on the 90th day compared to baseline. β-glucan ingestion in polypectomized patients significantly decreased the genotoxicity of their FW. Our findings suggest that β-glucan consumption could possibly provide protection against colon cancer development.