A combined coagulation–ultrafiltration method for enhanced separation of proteins and polyphenols. Issue 6 (12th April 2016)

• Record Type: Journal Article
• Authors: Yu, X.; Bogaert, L.; Hu, R.; Bals, O.; Grimi, N.; Vorobiev, E.
• Is Part Of: Separation science and technology. Volume 51:Issue 6(2016)
• Page Start: 1030
• Page End: 1041
• Publication Date: 2016-04-12
• Subjects: Coagulation -- combination methods -- rapeseed stem extracts -- separation of proteins and polyphenols -- ultrafiltration; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2016.1141957
• Languages: English
• ISSNs: 0149-6395

Abstract

ABSTRACT: This work aimed to separate proteins and polyphenols from rapeseed stem extracts. The results showed that coagulation with 40% w/w ethanol and ultra-filtration with 10 kDa membrane were two efficient methods for polyphenols purification. The purity of polyphenols increased from 80.3% to 97.0% after separation. The combination of ethanol coagulation with ultrafiltration permitted decreased the ethanol consumption from 40% to 10% w/w while maintaining high polyphenols purity. Significant protein loss at room temperature was observed during the stability test, which emphasizes the importance of elaborating a fast and effective separation method for rapeseed stem extracts.