The odontogenic differentiation of human dental pulp stem cells on hydroxyapatite-coated biodegradable nanofibrous scaffolds. Issue 14 (21st September 2016)

• Record Type: Journal Article
• Authors: Asghari, Fatemeh; Salehi, Roya; Agazadeh, Marziyeh; Alizadeh, Effat; Adibkia, Khosro; Samiei, Mohammad; Akbarzadeh, Abolfazl; Aval, Negar Abbasi; Davaran, Soodabeh
• Is Part Of: International journal of polymeric materials. Volume 65:Issue 14(2016)
• Page Start: 720
• Page End: 728
• Publication Date: 2016-09-21
• Subjects: Dental pulp stem cells -- differentiation -- nanofibers -- odontogenic -- regenerative medicine -- scaffold -- tissue engineering; Polymers -- Periodicals; Plastics -- Periodicals; 620.19205
• DOI: 10.1080/00914037.2016.1163564
• Languages: English
• ISSNs: 0091-4037

Abstract

ABSTRACT: The authors aimed to design nanofibrous (NF) scaffolds that facilitate odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (DPSCs) in vitro. For this purpose, hydroxyapatite (HA)–loaded poly (L-lactic acid)/poly (ϵ-caprolactone) (PLLA:PCL 2;1) blend NFs were prepared using the electrospinning method. Alizarin red activity and cell viability were evaluated by MTT assay, and SEM revealed the proliferation properties of NF scaffolds. QRT-PCR results demonstrated that HA-loaded PLLA/PCL can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that electrospun biodegradable PCL/PLA/HA has remarkable prospects as scaffolds for bone and tooth tissue engineering. GRAPHICAL ABSTRACT: